Educational scope: Laboratory documentation, analytical methods, and research recordkeeping only.
Chromatography provides separation context
HPLC separates detected components and records their response over time. Retention behavior can support comparison under controlled conditions, while a chromatogram alone does not fully establish molecular identity.
Mass spectrometry provides mass evidence
Mass spectrometry measures ions by mass-to-charge ratio. Agreement between expected and observed molecular-mass evidence can support identity when the instrument, method, calibration, and interpretation are appropriate.
Orthogonal evidence reduces ambiguity
Methods based on different scientific principles are often reviewed together because each has distinct strengths and limitations. The report should connect every result to the same sample and batch.
References and further reading
- ICH — Q2(R2) Validation of Analytical Procedures
- FDA — Analytical Procedures and Methods Validation
- Recommendations for peptide storage and handling in mass-spectrometry assays
Research-use notice
This material is educational and does not provide medical, dosing, administration, or human-use guidance.
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